Water Microbiology

Alyse Rose Microbiology Lab Bacterial strange March 25, 2013 Bacterial Unknown Report Each student was given an unknown bacterium to course out. I was given the unknown bacterium S38. Everybody is supposed to do altogether sorts of test to identify the bacteria. The first thing I did was smear my bacteria on a liquid average. I then proceeded to incubate the medium for 24-48 hours. 1. GRAM STAIN The near step I took in purpose my unknown bacteria was to gram stain it.This is utilise to diametricaliate the bacteria. The different staining reagents are crystal violet, grams iodine, acetone-alcohol, and grams safranin. Under the microscope it was a pink color, which intend Gram proscribe. Also, the shape was a rod. 2. KLIGERS IRON AGAR (KIA) The next step I did was the KIA bung. This is for the Negative bacteria to see if they are in the Enteriobacteriaceae group. There are many different results than can occur, like 1.Yellow slant/yellow of late- (A/A) glucose & milk sug ar are fermented if the butt has bubbles in it or is displaced, the furnish is gas positive. 2. Red slant/yellow deep- (K/A) glucose but not lactose is fermented 3. Red slant/black deep- (K/A) glucose but not lactose is fermented and H2S2 is produced. The deep is acid (yellow) 4. Red slant/ red deep- (K/NC) neither carbohydrate is fermented. Peptone is vitiated aerobically My result was a red slant and black deep. When is H2S2 is produced.Read Chapter 8 Microbial GeneticsThe deep is acid (yellow). Glucose was fermented and lactose wasnt. 3. UREASE HYDROLYSIS TEST This test is used to see if urea is used. Proteus can be determined because at that place fast urease activity. Proteus is the ability to utilize lactose. My test resulted in a positive result. It was a pink color. In my end result, my unknown bacteria is Proteus Vulgaris. Gram Stain appointed Gram Stain Negative gram stain KIARed slant/black deep Urease Test Urease Positive Urease Negative Proteus Vulgaris